Targeted mutagenesis by homologous recombination in D. melanogaster
نویسندگان
چکیده
منابع مشابه
Targeted mutagenesis by homologous recombination in D. melanogaster.
We used a recently developed method to produce mutant alleles of five endogenous Drosophila genes, including the homolog of the p53 tumor suppressor. Transgenic expression of the FLP site-specific recombinase and the I-SceI endonuclease generates extrachromosomal linear DNA molecules in vivo. These molecules undergo homologous recombination with the corresponding chromosomal locus to generate t...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 2002
ISSN: 0890-9369
DOI: 10.1101/gad.986602